Some bacteria contain a circular piece of DNA, known as a plasmid. This plasmid can be digested (cut open) by using specific enzymes known as restriction enzymes.
Once the plasmid is open, a gene can be attached and the plasmid closed to again form a circular piece of DNA, containing the foreign gene. This process is known as plasmid recombination.
Figure 1 shows a sequenced plasmid. Each colored arrow represents a known gene. Restriction enzymes will cut at recognition sites, certain nitrogenous base sequences.
Restriction enzyme sites are labeled, and restriction enzyme recognition sites were recorded and assigned a letter, a through. These data are recoded in Table 1.
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Question 1 of 5
1. Question
Question 1:
Based on the data in Figure 1 and Table 1, if a scientist combined the plasmid from this experiment with restriction enzymes SaII and NcoI, how many pieces would the plasmid be divided into?
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Question 2 of 5
2. Question
Question 2:
Using which of the following pairs of enzymes would result in the plasmid, shown in Figure 1, to be divided into two nearly equally-sized pieces?
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Question 3 of 5
3. Question
Question 3:
The scientist used another restriction enzyme, EcoRI, and mixed this with the plasmid. After an incubation period, he found that the plasmid was still intact. Which of the following answer choices best explains these results?
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Question 4 of 5
4. Question
Question 4:
Based on the information provided, using which of the following restriction enzymes would most likely result in a disruption of gene function?
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Question 5 of 5
5. Question
Question 5:
During cell replication, the bacterial DNA is copied. The scientist determined that GFP was the first gene to be copied, and that the process continued clockwise. Based on this information and Figure 1, which of the following would be the last gene replicated.
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