Science 44

Question 1:

Based on the data in Figure 1 and Table 1, if a scientist combined the plasmid from this experiment with restriction enzymes SaII and NcoI, how many pieces would the plasmid be divided into?

Question 2:

Using which of the following pairs of enzymes would result in the plasmid, shown in Figure 1, to be divided into two nearly equally-sized pieces?

Question 3:

The scientist used another restriction enzyme, EcoRI, and mixed this with the plasmid. After an incubation period, he found that the plasmid was still intact. Which of the following answer choices best explains these results?

Question 4:

Based on the information provided, using which of the following restriction enzymes would most likely result in a disruption of gene function?

Question 5:

During cell replication, the bacterial DNA is copied. The scientist determined that GFP was the first gene to be copied, and that the process continued clockwise. Based on this information and Figure 1, which of the following would be the last gene replicated.